Title

Identification of the product of toxoflavin lyase: Degradation via a baeyer-villiger oxidation

Document Type

Article

Publication Date

3-21-2012

Abstract

Toxoflavin (an azapteridine) is degraded to a single product by toxoflavin lyase (TflA) in a reaction dependent on reductant, Mn(II), and oxygen. The isolated product was fully characterized by NMR and MS and was identified as a triazine in which the pyrimidine ring was oxidatively degraded. A mechanism for toxoflavin degradation based on the identification of the enzymatic product and the recently determined crystal structure of toxoflavin lyase is proposed. © 2012 American Chemical Society.

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