Date of Award


Document Type


Degree Name

Master of Science

Degree Discipline



Kombucha SCOBY pellicle formed as a waste product in the kombucha tea fermentation industry can be used as a potential source for bacterial cellulose. The research compared four simple, scalable purification methods for the purification of this jelly-like cellulosic matrix containing bacteria, yeast cells, proteins, and polyphenols as impurities. The method using two successive 1.0 M NaOH washing at 90°C, followed by the treatment of 1.5 % (w/w) aq. NaOCl for 2h was the most effective method and gave the purified cellulose sample the highest Leucometer whiteness value of 81.4±4.8. The purified cellulose samples were analyzed by FT-IR, TGA, SEM, EDS, and water absorption at room temperature. After purified, the cellulose samples were pyrolyzed, and graphene oxide was directly produced at 600 to 800°C. The pyrolysis samples were analyzed by FT-IR, SEM, RAMAN, and ZETA potential.

Committee Chair/Advisor

Ananda S. Amarasekara


Prairie View A&M University


© 2021 Prairie View A & M University

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Date of Digitization


Contributing Institution

John B Coleman Library

City of Publication

Prairie View




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