Date of Award
Master of Science
Kombucha SCOBY pellicle formed as a waste product in the kombucha tea fermentation industry can be used as a potential source for bacterial cellulose. The research compared four simple, scalable purification methods for the purification of this jelly-like cellulosic matrix containing bacteria, yeast cells, proteins, and polyphenols as impurities. The method using two successive 1.0 M NaOH washing at 90°C, followed by the treatment of 1.5 % (w/w) aq. NaOCl for 2h was the most effective method and gave the purified cellulose sample the highest Leucometer whiteness value of 81.4±4.8. The purified cellulose samples were analyzed by FT-IR, TGA, SEM, EDS, and water absorption at room temperature. After purified, the cellulose samples were pyrolyzed, and graphene oxide was directly produced at 600 to 800°C. The pyrolysis samples were analyzed by FT-IR, SEM, RAMAN, and ZETA potential.
Ananda S. Amarasekara
Prairie View A&M University
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Date of Digitization
John B Coleman Library
City of Publication
Wang, D. (2020). Purification and Pyrolysis of Bacterial Cellulose from Kombucha Pellicle. Retrieved from https://digitalcommons.pvamu.edu/pvamu-theses/1480