Fabrication of BSA loaded poly (Caprolactone) (PCL)/ hydroxyapatite (HA) composite microsphere for tissue engineering application
In this study, PCL microsphere has been synthesised using the double emulsion technique and a freeze dry technique. Bovine Serum Albumin (BSA) was added as a model protein and Hydroxyapatite (HA) to enhance osteoconductivity properties of the microsphere. Poly (caprolactone) (PCL)-based microspheres were synthesized and characterized. Microspheres were produced with 5%, 10%, and 15% (w/v) PCL solution (30mL) by dissolving different amounts of PCL in dichloromethane (DCM). Microspheres produced from 5% (w/v) PCL concentration had an average diameter of 100 – 120 μm. Bovine serum albumin (BSA) (0.04% w/v) and 10% w/w of HA powder were added into the microspheres produced from 5% (w/v) PCL concentration. The microsphere was then characterized using different techniques and in vitro release study of BSA was conducted. Prepared microsphere showed good potential for bone tissue engineering.
Rashid, M., Naznin, S., & Salehhuddin, H. (2014). Fabrication of BSA loaded poly (Caprolactone) (PCL)/ hydroxyapatite (HA) composite microsphere for tissue engineering application. Retrieved from https://digitalcommons.pvamu.edu/medical-facpubs/41